MOULTRIE, Ga. — With the aid of a DNA expert, prosecutors on Wednesday morning sought to indicate the significance of Jeffery Peacock’s wardrobe change on the day five people were killed on Rossman Dairy Road.

Peacock was shown on video in the Hardee’s drive-through about 8:12 a.m. May 15, 2016, dressed in a green shirt with white lettering and a pair of khaki shorts. But by the time he arrived at 505 Rossman Dairy Road about 8:30, he was wearing a gray sleeveless shirt and cargo shorts, according to testimony and images entered into evidence on Monday, the first day of Peacock’s murder trial.

Georgia Bureau of Investigation crime scene specialist Amy Braswell testified later on Monday how she and a Colquitt County Sheriff’s Office investigator found the green shirt and khaki shorts stuffed between a speaker box and the seat of Peacock’s Chevrolet pickup truck.

Peacock had told law enforcement officers he’d gone to Hardee’s to get breakfast for himself and his friends at the Rossman Dairy Road house, but when he got back the house was engulfed in flames. His five friends — Jonathan Garrett Edwards, Ramsey Jones Pidcock and Aaron Reid Williams, all 21; Alicia Brooke Norman, 20; and Jordan Shane Croft, 22 — were found dead inside. A GBI autopsy later revealed they had been shot.

On Wednesday, Rachael Duke, who was a forensic biologist with the GBI Crime Lab in Macon at the time of the fire, testified that stains on the shirt and shorts were blood, and she was able to tie some of the stains to some of the people who died in the house.

Braswell provided Duke with clippings from the clothes rather than the entire articles, and Duke tested one or two stains on each clipping. Each was tested first to see if the stain was blood, and if so, Duke ran a test to see if she could determine whose DNA was on the fabric.

Defense attorney Allan Sincox pointed out that the DNA present didn’t necessarily come from the blood, and Duke agreed.

The first clipping she tested came from the lower back right leg of the shorts, and it contained two stains that Duke tested. Both tested positive for blood. One had DNA that matched up with Alicia Norman.

The other stain from there contained no human DNA, so Duke did a further test to determine if the blood was human. It was not. She said the GBI lab is not equipped to determine animal DNA, so no further tests were done.

Three dogs died in the same event that killed the five people, but Sincox also pointed out that Peacock was an avid hunter so the blood might have come from a game animal.

The second clipping from the shorts — taken from the “right butt area” — also produced two stains that were tested. Both showed the presence of blood, and a small quantity of human DNA was found. In one case, there wasn’t enough for any determination, she said. In the other, Duke could determine the DNA came from one person but there wasn’t enough of it for her to match it to any specific individual.

A final clipping from the shorts came from the right pocket area. It tested positive for blood, and Duke was able to isolate DNA from two people: Jones Pidcock and Jeffery Peacock himself.

Since the shorts belonged to Peacock, Duke said his DNA could be expected to be found there.

The green shirt produced two clippings. One, from the lower right front of the shirt, had the DNA of at least three people but the mixture was so complex the GBI couldn’t sort out whose it was. In fact, Duke initially thought there were four people’s DNA involved, but under the advice of her supervisor, she reported at least three because it was a more conservative and more defensible determination.

The other clipping from the green shirt contained the DNA of two people but there wasn’t enough to identify who they were.

Duke also tested a shirt that was found in the hallway of the burned house. It tested positive for blood and contained Jordan Croft’s DNA, she said.

Also on Wednesday, Dr. Moges Woldemariam, a veterinary pathologist at the University of Georgia’s Tifton campus, described the necropsies he performed on the three dogs who died at the residence.

Two were severely burned, and Woldemariam listed the cause of death as both severe burning and smoke inhalation. They were the dogs whose carcasses were found in the living room, one of the most heavily burned areas of the house.

The third dog was found under a truck outside. The truck had significant heat damage from the fire, according to statements earlier in the trial, but that isn’t what killed that dog.

Woldemariam described the dog’s skull as being crushed. Its face was burned severely but the rest of its body wasn’t.

He said in his opinion, the dog’s skull was “crushed between two hard surfaces,” in response to questions from defense attorney Jerry Word. Word was speculating that the dog may have been hit when the roof of the house collapsed and extricated itself from the rubble only to die outside from its injuries.